WebbThe pGEM®-T Vector size is 3000bp and the pGEM®-T Easy Vector size is 3,015bp. 6 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll … WebbRating: 5.0. Application Area: Cloning metastasis genes for expression and sequencing analysis. " The pGEM-T easy vector system is one of the best products we use for cloning. It has high efficiency for the genes we work with and purchase the system with the competent cells as we are guaranteed results. The step by step protocol is easy to ...

PCR Cloning T-Vector Cloning TA Cloning - Promega

WebbT vectors are linearized plasmids that have been treated to add T overhangs to match the A overhangs of the PCR product. PCR fragments that contain an A overhang can be … WebbFor full activity, add fresh S-adenosylmethionine (SAM). Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites … felix valencia

Doug MacMillan - Developer Support Engineer - Penumbra, Inc.

WebbIn linear algebra, a coordinate vector is a representation of a vector as an ordered list of numbers (a tuple) that describes the vector in terms of a particular ordered basis. An easy example may be a position such as (5, 2, 1) in a 3-dimensional Cartesian coordinate system with the basis as the axes of this system. Coordinates are always specified relative to an … WebbThe pGEM ® -T and pGEM ® -T Easy Vector Systems are convenient systems for the cloning of PCR products. The vectors are prepared by cutting the pGEM ® -5Zf (+) and pGEM ® -T Easy Vectors, respectively, with EcoR V … Webb19 feb. 2016 · We have demonstrated that GoTaq® Long PCR Master Mix successfully generated DNA fragments that could be ligated into pGEM®-T Easy Vector System without an A-tailing procedure, and with ligation efficiencies similar to those observed with the GoTaq® Green Master Mix. For details refer to Truman, A., Hook, B. and Wieczorek, D. félix v