WebUsingm Sephadex-DEAE column, the active fractions were eluted using 0.05 M Tris-HCl buffer containing 0.30 M NaCl at pH 7.5. The molecular weight of α-amylase was found to … WebIon exchange is probably the most frequently used chromatographic technique for separating and purifying proteins, polypeptides, nucleic acids, and other charged …
SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva
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Sephadex ion Data File exchange media Ion exchange …
WebBaMtx was purified using CM-Sephadex-C-25 ion-exchange resin and SDS-PAGE analysis showed a unique protein band of 13 kDa or 24 kDa under reducing or non-reducing conditions, respectively. cDNA sequence codified a 122-aa mature protein with high homology with other Lys49-PLA2s; modeled structure showed a N-terminal helix, a β-wing … WebA method and an apparatus suitable for a continuous chromatography process which only needs three separation columns, and a two-step process containing two chromatographic steps, in which the first chromatographic step (capture) is performed alternating and sequentially on two separation columns, the second chromatographic step (polishing) is … WebSP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. Well established strong cation exchanger. Based on … hernando school calendar 22-23