WebRSC Chemical Solutions WebN-Glycan Analysis InstantPC and 2-AB labeled N-glycans were separated by hydrophilic interaction liquid chromatography (HILIC) using an Agilent AdvanceBio Glycan Mapping column along with an Agilent 1290 Infinity II UHPLC system with in-line fluorescence detection (Table 1) coupled to an Agilent 6545 LC/Q-TOF mass spectrometer (Table 2). …

Hydrophilic Interaction Chromatography (HILIC)

http://rscbrands.com/ Web李爱峰， 闫业举， 邱江兵， 李 敏， 付艺蕾 (1. 中国海洋大学环境科学与工程学院， 山东 青岛 266100； 2. 中国海洋大学海洋环境与生态教育部重点实验室， 山东 青岛 266100) ctsfw textbooks

Hydrophilic Interaction Chromatography (HILIC)

WebSep 15, 2024 · The method provides a non-toxic alternative for derivatization in comparison to other common reducing agents that result in formation of toxic hydrogen cyanide in the reaction ( 18 ). The 2AB labeled analytes are chromatographed via HPLC using HILIC and detected by FLD. WebHILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase … Webby HILIC/FLD. Under the developed chromatographic conditions (Table 1), the InstantPC-labeled N-glycans from both EG2-hFc and rituximab resulted in well-resolved peaks for all major N-glycan species (Figures 2 and 3). The developed HILIC/FLD method for glycan profiling shows good reproducibility (data not shown). As shown in Figure 2A, the glycan ctsfy