WebApr 13, 2024 · For fluorescence imaging on TissueFaxs, a preview objective 2,5x (air) and for further acquisition an objective 40x (air) was used. In the acquisition mode Dapi filter was used as master channel. For Phalloidin FITC, the green filter (44FI) was used and the red filter (71HC) was used to visualize the marker. WebFITC/ Texas Red double-labeled tissue sections are analyzed and imaged with a Zeiss (Oberkochen, Germany) LSM 410 inverted confocal microscope with Zeiss Plan-Neofluar objectives. For visualization of FITC, an Ar/Kr 488/568 laser is used with 515-to 540-nm bandpass emission, and for visualization of Texas Red the same laser is used with a 590 ...
Fluorophores for Confocal Microscopy - Olympus
WebAnother benefit of using these probes together is the ability to measure fluorescence intensity fluctuations with a standard FITC/Texas red interference filter combination. … WebSolid State 488 Green FITC Alexa Fluor® 488 Yellow PE Orange PE-Texas Red® Red PerCP PE-Cy5a PerCP-Cy5.5 Infrared PE-Cy7 Solid State 532 or 561 Yellow PE Orange PE-Texas Red® Red PE-Cy5a Infrared PE-Cy7 Solid State 640 Red APCa Alexa Fluor® 647 Far Red Alexa Fluor® 700 Infrared BD APC-H7 APC-Cy7 Solid State 405 Green BD … im ok crying
Filters for Fluorescence In Situ Hybridization (FISH) Imaging
WebAlexa Fluor® dyes make brighter and more photostable conjugates than do fluorescein (FITC), Cy®3, Cy®5, Texas Red®, or allophycocyanin (APC) dyes. Alexa Fluor® 488 vs. … WebThe FITC-Texas Red dual excitation band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes FITC and Texas Red with … An excellent mechanism for rendering contrast in transparent specimens, … The Nikon Museum of Microscopy features many of the older microscopes and … Rat Sections. The humble rat has had an outsized impact on human history. In the … Contributing Authors. Kenneth R. Spring - Scientific Consultant, Lusby, Maryland, … Live-Cell Imaging. An increasing number of investigations are using live-cell imaging … Filter sets for DAPI, FITC, and TRITC or Texas Red. Ultraviolet Excitation. … This section explores new concepts in digital imaging technology and reviews … Contributing Authors. Philip C. Robinson - Department of Ceramic Technology, … MicroscopyU Contact Information. For all questions, comments, suggestions, or … The MicroscopyU digital image galleries present a wide spectrum of specimens … WebA single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5' or 3' end of oligos. imokhles sources ios 7.1.2