Cytof scrna-seq

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August undefined, 2024

WebMar 13, 2024 · While experimental and informatic techniques around single cell sequencing (scRNA-seq) are advanced, research around mass cytometry (CyTOF) data analysis has severely lagged behind. CyTOF data are dramatically different from scRNA-seq data in many aspects. This calls for the evaluation and development of computational methods … WebTo define the cell populations that drive joint inflammation in rheumatoid arthritis (RA), we applied single-cell RNA sequencing (scRNA-seq), mass cytometry, bulk RNA sequencing (RNA-seq) and flow cytometry to T cells, B cells, monocytes, and fibroblasts from 51 samples of synovial tissue from patients with RA or osteoarthritis (OA).

Single-Cell Analyses of Colon and Blood Reveal Distinct

WebDec 10, 2024 · This repository contains the R Markdown files with the analysis of CyTOF and scRNA-seq data corresponding to cohort 1 (Berlin) analysed in Georg et al. 2024 "Complement activation induces excessive T cell cytotoxicity in severe COVID-19". Additionally, here we include the necessary CyTOF data to reproduce this analysis. … WebFeb 3, 2024 · Using CyTOF and scRNA-seq, we described a similar heterogeneity of plaque CD4 + and CD8 + T cells, comprising activated, cytotoxic and resting cells. 31 In our study, we also found that resting T cells were enriched in blood and less represented in plaque from the same patient, indicating a specialized adaptive immune response in the … diabetes stomach problems

How Single-Cell Technologies Have Provided New Insights Into ...

WebFor instance, a combination of flow cytometry, CyTOF and scRNA-Seq was employed to analyze changes (pretreatment and post-treatment) in circulating B cells of patients with advanced melanoma who received combination ICB therapy or mono-ICB (anti-CTLA-4 or anti-PD-1). 49 It was found that a significant decline in overall B cell numbers … WebSep 2, 2024 · Introduction. Single-cell (sc) technologies, including flow cytometry (FACS), mass cytometry (CyTOF), cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), single-cell RNA sequencing (scRNA-seq), single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq), single-cell T cell receptor … WebJun 23, 2024 · The immune characteristic differences between active UC (UCa) and inactive UC (UCin) have not been completely explained. Mass cytometry (CyTOF) and single-cell … diabetes store coupons

Frontiers Single-cell analysis of the adaptive immune response to ...

Cross-tissue organization of the fibroblast lineage

WebAug 28, 2024 · Here, we use cytometry by time of flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) methodologies to identify a committed unipotent early-stage neutrophil progenitor (NeP) in adult mouse bone marrow. Importantly, we found a similar unipotent NeP (hNeP) in human bone marrow. Both NeP and hNeP generate only … cindy crosby actressWebDec 19, 2024 · Hashtag-enabled demultiplexing based on ubiquitous surface protein expression. We sought to extend antibody-based multiplexing strategies [16, 17] to scRNA-seq using a modification of our … diabetes stopping medication

"WebJan 11, 2024 · CyTOF distinguished NK cells and T cells more accurately, while scRNA-seq detected plasma cells that CyTOF markers did not cover (Figs. 2 and 3). Taken together, … We would like to show you a description here but the site won’t allow us. " - Cytof scrna-seq

Cytof scrna-seq

Janelia Research Campus - Hippocampus RNA-seq atlas

WebMucosal scRNA-seq identifies IL17A + T cells in UC and CyTOF Analysis Demonstrates Expansion of HLA-DR + CD38 + T Cell Subsets in IBD A. scRNA-seq of UC a subjects … WebApr 27, 2024 · Recently developed single-cell profiling technologies, such as single cell sequencing (scRNA-seq) and mass cytometry (CyTOF), provide critical biological …

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WebApr 13, 2024 · Utilizing single-cell RNA-Seq, ChiP-Seq, ATAC-Seq, RNA-Seq, RPPA, and mass cytometry (CyTOF) analyses, present pre-clinical studies elucidate gene-expression correlates of MI efficacy in AML cells ... WebApr 6, 2024 · New techniques using spatial proteomics [for example, mass cytometry imaging in tissue slices, or tissue cytometry by time of flight (tissue CyTOF)] would supplement flow cytometry data with the spatial localization of certain cell populations in a tissue, especially when combined with spatial transcriptomics, ideally integrated with …

WebDetermining the spatial organization and morphological characteristics of molecularly defined cell types is a major bottleneck for characterizing the architecture underpinning … WebOct 25, 2024 · scRNA-seq, CyTOF, and CITE-seq are increasingly used for evaluating cellular heterogeneity. Understanding their concordances is of great interest. To date, …

WebJul 15, 2024 · Winkels and colleagues used CyTOF to validate the 11 heterogeneous leukocyte populations identified with scRNA-seq of aortas from Apoe−/− mice fed a … WebJun 23, 2024 · Background: The pathogenesis of ulcerative colitis (UC) is closely related to immunity. The immune characteristic differences between active UC (UCa) and inactive UC (UCin) have not been completely explained. Mass cytometry (CyTOF) and single-cell RNA sequencing (scRNA-seq) were used to analyze the immune cells of UCa, UCin and …

WebFeb 18, 2024 · While experimental and informatic techniques around single cell sequencing (scRNA-seq) are advanced, research around mass cytometry (CyTOF) data analysis …

WebFurthermore, cytometry by time-of-flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) analyses were carried out to define the immune cell landscape of HCC. Not only did … cindy crouchWebscRNA-seq data are inherently integer count. CyTOF is also free from the drop-out issue that is commonly seen in scRNA-seq data(6–8) . On the other hand, more and more studies are now trying to generate matched CyTOF and scRNA-seq data for the single cells from the same research subjects(9–11) . diabetes stores near meWebFurthermore, cytometry by time-of-flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) analyses are carried out to define the immune landscape of HCC. It is found that 3-HAA treatment can significantly suppress tumor growth in the HCC model and alter the level of various cytokines in plasma. cindy crosta facebookWebThis vignette demonstrates how to map scRNA-seq and scATAC-seq data to Azimuth reference datasets directly in R, without the need to upload data into the web application. Reference-based mapping provides an attractive alternative to unsupervised analysis. When well-curated and annotated references are available, reference-mapping can rapdidly ... cindy croushoreWebCellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), developed by the New York Genome Center (NYGC), brings together surface protein phenotyping (similar to flow cytometry) and single-cell RNA sequencing (scRNA-seq) 1. This is achieved by using antibodies conjugated to oligonucleotides that can be captured in a similar way ... cindy croteauWebOct 25, 2024 · scRNA-seq, CyTOF, and CITE-seq are increasingly used for evaluating cellular heterogeneity. Understanding their concordances is of great interest. To date, this study is the most comprehensive … diabetes strategic frameworkWebMay 12, 2024 · We performed scRNA-seq on tumour and normal adjacent tissue (NAT) samples from three patients with pancreatic cancer and identified two subsets of fibroblasts ( n = 21,262 cells; Extended Data... cindy crouse obituary