WebFigure 5. The p24 complex can interact with COPI independently of Arf1 activation. ( A) erg6Δ mutant cells expressing HA-Gea1 and the COPI coat subunit Sec21-GFP were incubated with or without 100 µg/mL BFA (Brefeldin A) for 20 min. erg6Δ mutation was used to increase the plasma membrane permeability to BFA. WebDec 22, 2004 · The drug brefeldin A (BFA), an uncompetitive inhibitor of the exchange reaction that binds to an Arf–GDP–Arf GEF complex, stabilizes GBF1 on Golgi membranes. Using an in vivo assay to monitor …
Toxins Free Full-Text Arf6-Dependent Intracellular Trafficking of ...
WebJul 16, 2004 · stably associated with the Golgi but rather cycle rapidly on and off membranes. The drug brefeldin A (BFA), an uncompetitive inhibitor of the exchange reaction that binds to an Arf–GDP–Arf GEF complex, stabilizes GBF1 on Golgi membranes. Using an in vivo assay to monitor Arf1-GTP levels, we show that GBF1 exchange activity … WebIt is well documented that the brefeldin A (BFA)-inhibited guanine nucleotide exchange factor (GEF), named BIG, regulates vesicle budding at the trans-Golgi network (TGN) and recycling endosomes through activating the ADP-ribosylation factor (ARFs). ... Besides ARF1, both BIG5 and BIG1-BIG4 can activate ARFA and possibly ARFB, which is not ... most activity
Brefeldin A ≥99%(HPLC) Selleck ATPase inhibitor
WebNov 20, 2024 · The drug brefeldin A (BFA) has been instrumental in the study of dynamic mechanisms underlying Golgi structure and function 26. BFA blocks activation of the small G protein Arf1, which results... WebJul 31, 2024 · To better understand the mode of ARF1 activation in vivo, Brumm et al. (2024) ... between GNOM and endogenous ARF1 were detected only at the membrane upon stabilization with the trafficking inhibitor Brefeldin A (BFA). Together, these experiments demonstrate that critical steps of GNOM-dependent ARF activation occur at … WebBackground. Brefeldin A (BFA) is an inhibitor of ATPase with IC50 value of 0.2 μM [1] ATPase is a chemical enzyme which is essential in the ADP/ATP exchange which … most active ws